滇池、红枫湖沉积物中总磷、分态磷及生物硅形态与分布特征

通过对不同富营养化程度的湖泊（滇池及红枫湖）沉积柱剖面进行不同形态磷以及生物硅含量分析,对比了磷及生物硅的沉积分布特征,并探讨了其与湖泊富营养化演化的关系。结果表明：滇池沉积物中碎屑磷和有机磷在总磷中占50.5%～80.8%,是影响总磷趋势的主要原因;红枫湖中有机磷对总磷的贡献较大,占总磷的53%～60.9%;且两湖各形态磷均随深度的增加而减少;两湖总磷、有机磷、铝磷以及滇池沉积柱中的碎屑磷等的快速增长,表明其主要来源于人为污染;生物硅含量呈现从下层沉积物向上逐渐增加,在上层沉积物中逐渐减少的趋势;总磷、有机磷、铁结合态磷、铝结合态磷、弱结合态磷以及生物硅都能较好地反映富营养化污染的演变和现状。     

甘南草原主要草食动物的食性及其生态位特征

家畜放牧系统中草食动物食性及其生态位特征的认识对草地管理有重要的现实指导意义。本文采用粪便和胃内容物显微组织分析法,分析了甘南草原4种主要草食动物牦牛(Bos mutus)、藏羊(Ovis aries)、喜马拉雅旱獭(Marmota himalayana)和高原鼢鼠(Eospalax baileyi)秋季的食性及食物生态位重叠程度。结果表明,4种动物的食物资源谱存有较大差异,禾草科的发草(Deschampsia caespitosa)和莎草科的扁穗草(Brylkinia compressus)是藏羊和牦牛食物结构的主体,而喜马拉雅旱獭的食谱中绝大部分是鹅绒委陵菜(Potentilla anserina)和克氏针茅(Stipa captillata),高原鼢鼠采食具有发达根茎的美丽风毛菊(Sausserua superba)、鹅绒委陵菜、蒲公英(Taraxacum mongolicum)及垂穗披碱草(Elymus nutans)。牦牛和藏羊的食物生态位宽度显著高于喜马拉雅旱獭和高原鼢鼠。食物生态位重叠度以藏羊和牦牛最高,其次为喜马拉雅旱獭和高原鼢鼠,重叠度最低的是藏羊和高原鼢鼠。甘南草原4种主要草食动物的食性及其生态位特征的分析可为优化当地草地管理策略提供重要依据。     

柑橘黄龙病赣南脐橙内生菌种群结构分析

【目的】分析赣南脐橙黄龙病植株和健康植株叶片内生菌,对比不同培养条件下培养出的内生菌,为筛选出对柑橘黄龙病原菌有影响的伴生菌奠定理论基础。【方法】通过PCR方法对脐橙中黄龙病菌进行验证,并基于16S r RNA基因高通量测序技术对患病与健康赣南脐橙叶片内生菌以及不同培养基富集培养后的内生菌进行多样性分析。【结果】所采集样品中有5株患病株,5株健康株。5株病株中共同含有的细菌属有13个,其中7个在5株健株中也共同存在。Defluviicoccus属和Granulicella属在病健株植物中都是优势菌属,且在健株中的平均含量高于病株。病株与健株的样品相似度存在明显界线。富集培养后不同样本和不同培养基中菌属分布不同。肠杆菌属(Enterobacter)、短小杆菌属(Curtobacterium)、假单胞菌属(Pseudomonas)和泛菌属(Pantoea)得到了大量富集,不动杆菌属和沙雷氏菌属等9个菌属富集量较少。另外,培养和未培养各样本间未分类菌(Unclassified)含量差异也较大。【结论】赣南脐橙患病植株和健康植株叶片内生菌有着明显差异,黄龙病菌的存在改变了脐橙叶片原有内生细菌的菌群结构。从活体植物组织内直接检测才能得到真正的植物内生菌群落分布情况。通过分析菌群的差异,有望找到与柑橘黄龙病菌生长相关的伴生菌。     

The role of biomass allocation strategy in diversity loss due to fertilization

Proposed mechanisms for explaining biodiversity loss due to fertilization include interspecific competition and assemblage-level thinning. The interspecific competition hypothesis (ICH) assumes a link between population changes and species competitive ability, which is related to functional traits such as biomass allocation patterns. Based on a 2-year fertilization experiment in an alpine meadow on the Tibetan Plateau, we attempted to explore the relationships between individual and population responses. Individual response was measured by changes in plant biomass and biomass allocation, and population response was estimated by changes in species abundance. The results suggested that following fertilization (1) changes in individual biomass differ among species and functional groups, (2) reproductive allocation tends to decrease for all species whereas leaf allocation generally increases for grasses but decreases for forbs, (3) a strong positive correlation exists between species relative abundance change and individual biomass response, and (4) species relative abundance change has a positive correlation with leaf allocation response, a negative correlation with stem allocation response, and no significant correlation with reproductive allocation response. We conclude that the individual biomass responses and biomass allocation strategy can partly explain diversity loss due to fertilization, a result consistent with the ICH.     

Impact of land use changes on mountain vegetation

Abstract. In this study the impact of land use changes on vegetation in the sub‐alpine‐alpine belt is analysed. The study sites (4.7 km²) are located in the Passeier Valley (South Tyrol, Italy), at an elevation of 1500–2300 m a.s.l. The whole study area was used for hay‐making ca. 60 yr ago. Today, part of the meadows are more intensively used, while other parts have been converted to pasture or have been abandoned. We analysed the reasons for these land use changes and the effects on vegetation with a Geographical Information System and geostatistical analysis. The result of these analyses are: (1) Current land use is mainly controlled by the degree of accessibility for vehicles. Accessible areas are being used more and more intensively, while poorly accessible areas are being abandoned or used as pasture. (2) Current vegetation is highly determined by current land use. Particular vegetation units can be assigned to each form of land use. (3) Succession starts immediately after abandonment. Depending on altitude, succession proceeds at different speeds and with different numbers of stages. Hence the type of vegetation indicates the time passed since abandonment. (4) Land use changes lead to characteristic changes in vegetation; they are considered to be the most important driving force for vegetation change. (5) Measures of intensification and abandonment of extensively used areas both lead to a decrease in the number of species.     

Nest defence in the meadow pipit <Emphasis Type="Italic">Anthus pratensis</Emphasis>: the influence of renesting potential

The reproductive value hypothesis predicts that the level of nest defence is determined by the expected chance of offspring to survive until reproduction, and by the reproductive potential of the parents. Rates of survival from one breeding season to the next are low in small passerines, and their residual reproductive potential strongly declines as the current breeding season terminates. Therefore, we can expect that parents which have only one breeding attempt per season should defend their nests more intensively than parents with a possibility to renest. We studied nest defence in populations of meadow pipit (Anthus pratensis) breeding in Norway and the Czech Republic, differing in renesting potential. To simulate the threat from a predator, we placed a stuffed stoat (Mustela erminea) first 5 m and then 1 m away from a nest with nestlings. Parents increased or kept nest defence constant when the stoat approached their nests in Norway and, during a breeding season shortened by severe weather, in the Czech Republic (when renesting potential was limited). Parents decreased nest defence when the stoat approached the nest during “normal” breeding seasons in the Czech Republic (when renesting was common). These findings give support to the reproductive value hypothesis.     

Functional interpretation of archaeobotanical data: Making hay in the archaeological record

Hay malting and hay meadows have long been of fundamental ecological, economic and social importance in temperate Europe. A variety of archaeological sources suggests that hay making may date back to the Iron Age, but direct archaeobotanical evidence for this practice is problematic. Past grassland communities are imperfectly represented and preserved in archaeobotanical assemblages, and ancient meadow and pasture communities may not resemble present-day communities in terms of management practices or botanical composition. This paper explores the potential of FIBS (Functional Interpretation of Botanical Surveys) in the archaeobotanical investigation of ancient grassland management. The botanical composition of present-day grassland communities was analysed in terms of functional attributes (e.g. canopy height) relevant to cutting, grazing and habitat productivity. The utility of these attributes for distinguishing between present-day meadow, pasture and unmanaged grassland communities has been evaluated. Similar analyses were performed on archaeobotanical data from Neolithic to post-Medieval northwestern and central Europe. Functional shifts over time, interpreted in the light of the functional analysis of modern grassland, suggest that hay-making was well established by the Iron Age. Avenues are suggested for the refinement and further development of the FIBS methodology in the archaeobotanical investigation of grassland management.     

A linkage map of meadow fescue (<Emphasis Type="Italic">Festuca pratensis</Emphasis> Huds.) and comparative mapping with other Poaceae species

A genetic linkage map has been constructed for meadow fescue (Festuca pratensis Huds.) (2n=2x=14) using a full-sib family of a cross between a genotype from a Norwegian population (HF2) and a genotype from a Yugoslavian cultivar (B14). The two-way pseudo-testcross procedure has been used to develop separate maps for each parent, as well as a combined map. A total number of 550 loci have been mapped using homologous and heterologous RFLPs, AFLPs, isozymes and SSRs. The combined map consists of 466 markers, has a total length of 658.8 cM with an average marker density of 1.4 cM/marker. A high degree of orthology and colinearity was observed between meadow fescue and the Triticeae genome(s) for all linkage groups, and the individual linkage groups were designated 1F–7F in accordance with the orthologous Triticeae chromosomes. As expected, the meadow fescue linkage groups were highly orthologous and co-linear with Lolium, and with oat, maize and sorghum, generally in the same manner as the Triticeae chromosomes. It was shown that the evolutionary 4AL/5AL translocation, which characterises some of the Triticeae species, is not present in the meadow fescue genome. A putative insertion of a segment orthologous to Triticeae 2 at the top of 6F, similar to the rearrangement found in the wheat B and the rye R genome, was also observed. In addition, chromosome 4F is completely orthologous to rice chromosome 3 in contrast to the Triticeae where this rice chromosome is distributed over homoeologous group 4 and 5 chromosomes. The meadow fescue genome thus has a more ancestral configuration than any of the Triticeae genomes. The extended meadow fescue map reported here provides the opportunity for beneficial cross-species transfer of genetic knowledge, particularly from the complete genome sequence of rice.Communicated by P. Langridge     

Drivers of local densities of endangered Lycaena helle butterflies in a fragmented landscape

Due to their specialised habitat requirements, butterflies are particularly vulnerable to habitat loss and fragmentation. Understanding the drivers of local abundances of species is essential for their effective conservation in fragmented landscapes. We investigated factors affecting population densities of an endangered European butterfly, the Violet Copper (Lycaena helle), occurring in a small metapopulation near the city of Kraków, southern Poland. The environmental parameters tested as predictors of the local densities of the species included both the variables associated with spatial structure of habitats such as patch sizes, their isolation and fragmentation as well as those potentially reflecting habitat quality. Patch area and vegetation height turned out to be the only factors significantly influencing L. helle densities, both having a positive effect. The positive impact of patch area is a bit surprising, since its relationship with population densities is typically negative in butterflies. In our study system it is likely to derive from source-sink dynamics as the smaller habitat patches are apparently too small to sustain viable local populations. In turn, the positive influence of vegetation height implies that the ongoing succession does not deteriorate the quality of the recently abandoned meadows yet, whereas higher turf may provide better sheltering places. The loss of almost half of L. helle habitat patches in the study area in recent years is alarming. However, its inclusion into the Natura 2000 system should help to conserve the species as long as this act is followed by proper management of its habitats.